Applied molecular genetics
John Wiley, 1999/04/13 - 293 ページ
"Well-written, up-to-date and very comprehensive. The writing is confident, clear and accessible throughout. . . . Miesfeld has done a wonderful job of presenting contemporary molecular genetic strategies in an accessible and enjoyable style."-Paul A. Krieg, University of Texas.
Applied Molecular Genetics explains the key biochemical and cell biological principles behind some of today's most commonly used applications of molecular genetics, using clear terms and well- illustrated flow schemes. The book is divided into several sections and moves from basic to advanced topics while providing a concise overview of fundamental concepts in modern biotechnology. Each chapter concludes with a Laboratory Practicum describing a hypothetical research objective and the sequence of steps that are most often used to investigate biological questions using molecular genetic methods. In addition, the book provides informative summaries of the latest advances in molecular genetics, using attractive illustrations and a comprehensive reference list. Representative topics include:
* Genomic mapping using single nucleotide polymorphisms.
* Creating cell-specific gene knockouts in transgenic mice.
* Animal cloning methodologies based on whole nuclear transfer.
* Pharmacogenetic applications of DNA microarray technology.
This text introduces the use of Internet resources through the World Wide Web as a powerful new tool in molecular genetic research. Seven appendices are included in the book, providing a convenient resource for properties of nucleic acids, protein and restriction enzymes, a description of common E. coli genetic markers and gel electrophoresis parameters, as well as a list of useful Internet sites.
A must-have guide for all instructors and students in the fields of modern biochemistry, cell biology, and genetics, Applied Molecular Genetics is an ideal reference for anyone needing a clear, concise source of up-to-date information on this rapidly changing field.
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DNA Metabolizing Enzymes
Identifying the transcriptional start site of a gene
LABORATORY TOOLS FOR MOLECULAR
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Acad Acids Res activity agarose analysis annealing antibody applied molecular genetics assay bacterial bacteriophage biochemical Biol cDNA cloning cDNA library cDNA synthesis cell lines cell-specific chromosome cleavage cloning vector coding sequence coli contain denaturation detect developed digested DNA binding DNA fragments DNA polymerase DNA sequence DNasel double-strand EcoRI encoding eukaryotic expression vectors Figure function fusion proteins gel electrophoresis gene expression gene transcript genomic DNA human genome hybridization identify infection insert isolated laboratory lacZ ligation mammalian cells marker membrane method molecular genetic mouse mRNA mutagenesis mutations Natl Northern blot nucleic acid nucleotide oligo oligonucleotide PCR products phage plasmid plasmid vector plate pool primer pairs probe Proc promoter purified receptor recombinant region replication reporter gene restriction enzyme retroviral RNase RT-PCR RTase samples screening specific strains strand cDNA strategy target template transcription factor transfection transgenic viral vitro vivo yeast