Advanced Techniques in Soil MicrobiologyAjit Varma, Ralf Oelmüller Springer Science & Business Media, 2007/07/25 - 427 ページ "Advanced Techniques in Soil Microbiology" presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants. This volume of state-of-the-art, practice oriented methods will be of great use both to the first-timer and to the experienced scientist. |
目次
Functional Genomic Approaches for Mycorrhizal Research | 17 |
Automated Fluoroscence Sequencing and Troubleshooting 35 | 34 |
References | 50 |
Laboratory Practice for the Production of Polyclonal | 73 |
Modern Techniques for Analyzing Immunological Responses 93 | 92 |
Transcriptome Analysis | 111 |
a Tool for Functional Validation of Novel Genes 133 | 132 |
Techniques for Biomolecular | 145 |
CoCultivation with Sebacinales | 247 |
a Forgotten Tool | 271 |
of Fungal Trehalose Metabolism in Symbiosis | 277 |
Application of TerminalRestriction Fragment Length | 295 |
Molecular Symbiotic Analysis Between Arabiopsis thaliana | 306 |
in A thaliana which are Regulated by P indica | 317 |
22 | 343 |
Infested Roots | 350 |
Extraction and Identification | 155 |
References | 166 |
12 | 181 |
Measurement of Microbial Biomass and Activity in Soil | 201 |
ImmunoTechnology for the Localization of Acid Phosphatase | 212 |
15 | 237 |
References | 360 |
Research Methods in Arbuscular Mycorrhizal Fungi | 377 |
FieldTrialsofBioinoculants | 397 |
References | 412 |