The Zebrafish: Genetics, Genomics and InformaticsElsevier, 2004/12/30 - 630 ページ This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to the zebrafish community. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Illustrates many techiques in full-color * Summarizes the Zebrafish Genome Project |
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allele analysis antisense assay aVecting bacteria Biol buVer cDNA CELL BIOLOGY chromosome contigs cryopreservation culture Danio rerio database detected developmental diVerent diVerentiation diYcult duplication eggs Ekker embryos eVects exon expression patterns eYcient F1 fish females fertilization filters fluorescent fragments function gene expression gene trap genetic Genome Res genome sequence genomic DNA germline GFP expression heterozygous homozygous human hybridization HypNA-pPNA I-SceI identified incubation injected insertions laboratory lines males markers maternal-eVect medaka meganuclease METHODS IN CELL microarray microinjection Molecular Morpholino mRNA mutagenesis mutagenized mutations Natl NCBI oligonucleotides orthologs panel phenotype plasmid polymorphic positional cloning primers probe protein protocol recombination region RH mapping samples screen solution specific sperm synteny tanks target tissue transcription transgenesis transgenic transposase transposon vector vertebrate visual vitro Westerfield wild-type zebrafish zebrafish embryos zebrafish genome ZFIN