Manual of Techniques in Insect Pathology

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Lawrence A. Lacey
Academic Press, 1997/02/27 - 409 ページ
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.
The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.
This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.
This book will be an essential laboratory reference for insect pathologists.

Features include:
* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
* Details of the practical knowledge needed by beginners to apply the techniques
* Chapters written by an international group of experts
* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
* Provides extensive supplemental literature and recipes for media, fixatives and stains
 

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目次

Chapter II Viruses
17
Identification isolation culture and preservation of entomopathogenic bacteria
55
Laboratory bioassay of bacteria against aquatic insects with emphasis on larvae of mosquitoes and black flies
79
Bioassay of Bacillus thuringiensis against lepidopteran larvae
91
Chapter III4 Bacteria of soilinhabiting insects
101
Chapter IV Research methods for entomopathogenic protozoa
117
Identification
153
Entomophthorales
187
Preservation of cultures
269
Chapter VI Techniques in insect nematology
281
Chapter VII Testing the pathogenicity and infectivity of entomopathogens to mammals
325
Preparations of entomopathogens and diseased specimens for more detailed study using microscopy
337
Fluorescence microscopy
355
Chapter VIII3 The application of molecular techniques to insect pathology with emphasis on entomopathogenic fungi
367
Index
395
Color Plate Section
411

Hyphomycetes
213
Oomyeetes and Chytridiomycetes
251

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著者について (1997)

Dr. Lacey earned his PhD in 1978 and has been internationally active working in medical entomology, insect pathology, and microbial control of economically and medically important insects. He conducted research on the biology and microbial control of dozens of insects in the US, Amazon Basin, West Africa in the Azores, throughout Europe, the Middle East and Western and Southeast Asia and the Pacific Northwest of the USA. In his 40 year career he has written or co-authored nearly 150 refereed papers in medical entomology, agricultural entomology, and insect pathology, and edited and co-edited seven books, including the Manual of Techniques in Invertebrate Pathology, second edition and Microbial Control of Insect and Mite Pests: From Theory to Practice.

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