GB 5009.212-2016 Translated English of Chinese Standard. GB 5009.212-2016: National Food Safety Standard - Determination of Diarrheic Shellfish Poison in Shellfish [Buy--download True-PDF in 3-second: https://play.google.com/books > Sign in > This BOOK > "3-dots" > Export > Save as "*.pdf"]https://www.chinesestandard.net, 2019/06/18 - 22 ページ This Standard specifies mouse biology method, enzyme linked immunosorbent assay (ELISA method) and liquid chromatography - tandem mass spectrometry for the determination of diarrheic shellfish poison in shellfish. In this Standard, mouse biology method and enzyme linked immunosorbent assay (ELISA method) are applicable to the determination of diarrheic shellfish poison in shellfish and shellfish products; liquid chromatography - tandem mass spectrometry is applicable to the determination of diarrheic shellfish poison okadaic acid (OA), dinophysistoxins-1 (DTX-1) and dinophysistoxins-2 (DTX-2) in edible part of shellfish and shellfish products (excluding salted products). |
目次
| 3 | |
| 4 | |
| 5 | |
Expression of Analytical Result | 7 |
Enzyme Linked Immunosorbent Assay ELISA method | 8 |
Instruments and Equipment | 9 |
Analytical procedures | 10 |
Expression of Analytical Result | 11 |
Liquid Chromatography Tandem Mass Spectrometry | 12 |
Instruments and Equipment | 14 |
Expression of Analytical Result | 18 |
Precision | 19 |
Appendix A Evaluation Technical Parameters of Commercial Kit | 20 |
Appendix B Virulence Factor of Diarrheic Shellfish Poison | 21 |
Multiple Reaction Monitoring Chromatogram of Diarrheic Shellfish Poison Standard Solution | 22 |
多く使われている語句
accordance with Formula accordance with Table accurately weigh-take acetone acetonitrile ammonium formate anhydrous ether assay ELISA method chromatographic purity concentration of diarrheic constant volume cross reaction rate Determination of Diarrheic diarrheic shellfish poison Diluent Dinophysistoxins-2 DTX-2 Disodium hydrogen phosphate enzyme linked immunosorbent enzyme marker Homogenize Hydrochloric acid immunosorbent assay ELISA linked immunosorbent assay liquid chromatography mass concentration measure-take methanol solution mL of methanol mL of water Mobile phase Mouse Biology Method ng/mL Okadaic acid OA open the shell PBS solution Poison in Shellfish poison standard solution Potassium dihydrogen phosphate r/min reach a constant rinse room temperature round-bottomed flask Sample Extraction sample solution series working solution shell meat shellfish poison standard SN/T Sodium hydroxide solid phase extraction solution 2.5 mol/L solution hole solvent control group standard substance standard working solution Sulfuric acid tandem mass spectrometry test group test stock solution Tween-60 saline solution various diarrheic shellfish Virulence Factor water to dilute