Manual of Techniques in Insect PathologyLawrence A. Lacey Academic Press, 1997/02/27 - 409 ページ Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains |
目次
17 | |
55 | |
Laboratory bioassay of bacteria against aquatic insects with emphasis on larvae of mosquitoes and black flies | 79 |
Bioassay of Bacillus thuringiensis against lepidopteran larvae | 91 |
Chapter III4 Bacteria of soilinhabiting insects | 101 |
Chapter IV Research methods for entomopathogenic protozoa | 117 |
Identification | 153 |
Entomophthorales | 187 |
Preservation of cultures | 269 |
Chapter VI Techniques in insect nematology | 281 |
Chapter VII Testing the pathogenicity and infectivity of entomopathogens to mammals | 325 |
Preparations of entomopathogens and diseased specimens for more detailed study using microscopy | 337 |
Fluorescence microscopy | 355 |
Chapter VIII3 The application of molecular techniques to insect pathology with emphasis on entomopathogenic fungi | 367 |
Index | 395 |
Color Plate Section | 411 |
多く使われている語句
acid agar anisopliae assay Bacillus thuringiensis bacteria bassiana bioassay buffer cadavers centrifugation Chapter Coelomomyces concentration conidia conidiogenous conidiogenous cells conidiophores conidium containing counting coverslip culture cuticle density difficult dilution Diptera disease distilled water dosage dose ento Entomol entomopathogenic fungi entomopathogenic Hyphomycetes Entomophthorales ethanol extract field Figure filter paper final first fixative flask fluorescence fungal fungi fungus gene germination giganteum growth host hyphal bodies Hyphomycetes identification incubated influence inoculated inoculum insect pathology Invertebr isolation laboratory Lagenidium Lagenidium giganteum larvae liquid medium method microbial microscope microsporidia mopathogenic mosquito larvae MPCA nematodes nutrient organisms parasite pathogen Pathol Petri dish Plate popilliae preparation procedure production propagules protein protoplasts protozoa resting spores Rinse sample slide soil solution species specific specimens sphaericus sporulation stain sterile storage sufficient surface suspension techniques temperature tion tissue tube Undeen usually vector virus viruses vitro zoospores